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Coding

Part:BBa_J45011:Design

Designed by: Stephen Payne   Group: iGEM2006_MIT   (2006-06-07)


PAL (Phenylalanine Ammonia-lyase) catalyzes the conversion of phenylalanine to cinnamic acid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2508
    Illegal BamHI site found at 476
    Illegal XhoI site found at 551
    Illegal XhoI site found at 588
    Illegal XhoI site found at 651
    Illegal XhoI site found at 669
    Illegal XhoI site found at 804
    Illegal XhoI site found at 1005
    Illegal XhoI site found at 1248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1164
    Illegal NgoMIV site found at 1471
    Illegal NgoMIV site found at 1698
    Illegal NgoMIV site found at 1841
    Illegal NgoMIV site found at 2231
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 648
    Illegal BsaI site found at 1035
    Illegal BsaI site found at 1041
    Illegal BsaI site found at 1611
    Illegal BsaI site found at 1975
    Illegal BsaI.rc site found at 2342
    Illegal SapI site found at 995


Design Notes

The purpose of this part would be to develop cinnamic acid from natural food for E. coli. From there, if we can find the SAM:CCMT part, we could develop a cinnamon smell from E. coli merely from the cells eating their natural food.


Source

If we received such a part, it would probably be donated by Faulkner, et al. If not, the part would need to be modified to successfully express it in E. coli.

References